3D superresolution microscopy on the immunological synapse at isotropic precision below 15 nm

T-cells are part of our adaptive immune system and responsible for recognition antigens in bodies. T-cell activation is triggered upon binding receptor (TCR) to major histocompatibility complex loaded with antigenic peptide (pMHC) which presented on the surface antigen presenting cells (APC). According kinetic segregation model activation, topography plays a large role process. In this study we have applied 3D superresolution method spatial organization within immunological synapse isotropic localization precision below 15 nm.